PAÜ .:. Eğitim Öğretim Bilgi Sistemi

COURSE INFORMATION

Course Code	Course Title	L+P Hour	Semester	ECTS
TIB 514	INTRODUCTION TO GENE ENGINEERING	2 + 1	2nd Semester	6

COURSE DESCRIPTION

Course Level	Doctorate Degree
Course Type	Elective
Course Objective	To aim the fundamental principles of genetic engineering at master's level
Course Content	Fundamental principles of genetic engineering, methods used in genetic enginnering with advantages and disadvantages, to make new synthesis with the knowledge learned in this course
Prerequisites	No the prerequisite of lesson.
Corequisite	No the corequisite of lesson.
Mode of Delivery	Face to Face

COURSE LEARNING OUTCOMES

1	Define the fundamental principles of recombinat construction and gain experience in this field
2	Explain fundamental principles of gene transfer and selection of recombinant clones

COURSE'S CONTRIBUTION TO PROGRAM

	PO 01	PO 02	PO 03	PO 04	PO 05	PO 06	PO 07	PO 08	PO 09	PO 10	PO 11
LO 001	3	2	5	5	4	5	1	1	3	5	5
LO 002	4	4	5	5	5	5	3	3	4	5	5
Sub Total	7	6	10	10	9	10	4	4	7	10	10
Contribution	4	3	5	5	5	5	2	2	4	5	5

ECTS ALLOCATED BASED ON STUDENT WORKLOAD BY THE COURSE DESCRIPTION

Activities	Quantity	Duration (Hour)	Total Work Load (Hour)
Course Duration (14 weeks/theoric+practical)	14	2	28
Hours for off-the-classroom study (Pre-study, practice)	14	2	28
Mid-terms	1	15	15
Laboratory	14	1	14
Final examination	1	35	35
Presentation / Seminar Preparation	6	6	36
Total Work Load ECTS Credit of the Course			156 6

COURSE DETAILS

Select Year

Course Details

Course Code	Course Title	L+P Hour	Course Code	Language Of Instruction	Course Semester
TIB 514	INTRODUCTION TO GENE ENGINEERING	2 + 1	1	Turkish	2021-2022 Spring

Course Coordinator	E-Mail	Phone Number	Course Location	Attendance
Prof. Dr. İBRAHİM AÇIKBAŞ	iacikbas@pau.edu.tr		KKM A0318	%

Goals		To aim the fundamental principles of genetic engineering at master's level
Content		Fundamental principles of genetic engineering, methods used in genetic enginnering with advantages and disadvantages, to make new synthesis with the knowledge learned in this course

Topics

Weeks	Topics
1	concept of the recombinant DNA and genetic engineering
2	Restriction endonucleases and enzymes
3	vectors
4	lab work
5	E.coli and competent cells
6	DNA isolation and purification
7	Lab work
8	midterm exam
9	DNA transfection methods
10	Recombinant selection
11	DNA elektroforesis
12	Lab work
13	DNA libraries
14	Lab work

Materials

Materials are not specified.

Resources

Resources	Resources Language
Sambrook J., Fritsch E.F., Maniatis T., Molecular cloning: A laboratory manual. 2nd ed. Cold Spring Harbour Laboratory Press, New York, 1989, Vol 1-3	English
A CECCARELLI, GENETIC ENGINEERING (Medical Perspectives Series), Garland Science	English

Course Assessment

Assesment Methods	Percentage (%)	Assesment Methods Title
Final Exam	50	Final Exam
Midterm Exam	50	Midterm Exam

L+P: Lecture and Practice
PQ: Program Learning Outcomes
LO: Course Learning Outcomes

Prospective Student