PAÜ .:. Eğitim Öğretim Bilgi Sistemi

COURSE INFORMATION

Course Code	Course Title	L+P Hour	Semester	ECTS
KIM 514	PROTEIN SPECIFICATION METHODS	3 + 0	1st Semester	7,5

COURSE DESCRIPTION

Course Level	Master's Degree
Course Type	Elective
Course Objective	To provide information about understanding of methods of protein specification.
Course Content	General introduction of protein synthesis, Purity determination methods, Size molecular weight and subunit analysis, Aminoacid analysis, peptide map, cofactor analysis and protein modifications analysis, crystallization methods, radioactive labelling methods, immunological anaysis (Western blot), structure anaysis
Prerequisites	No the prerequisite of lesson.
Corequisite	No the corequisite of lesson.

COURSE LEARNING OUTCOMES

1	Learns the concepts of amino acid, peptide and protein.
2	Learns the qualitative and quantitative determination methods of proteins.
3	Understands the principles of the methods used in the isolation and purification of proteins and purity tests.
4	Learns how to perform sequence analysis of proteins. Understands how to identify proteins using chromatography-mass spectroscopy techniques.

COURSE'S CONTRIBUTION TO PROGRAM

ECTS ALLOCATED BASED ON STUDENT WORKLOAD BY THE COURSE DESCRIPTION

Activities	Quantity	Duration (Hour)	Total Work Load (Hour)
Course Duration (14 weeks/theoric+practical)	14	3	42
Hours for off-the-classroom study (Pre-study, practice)	14	4	56
Mid-terms	1	30	30
Final examination	1	32	32
Report / Project	5	7	35
Total Work Load ECTS Credit of the Course			195 7,5

COURSE DETAILS

Select Year

Course Details

Course Code	Course Title	L+P Hour	Course Code	Language Of Instruction	Course Semester	Mode of Delivery
KIM 514	PROTEIN SPECIFICATION METHODS	3 + 0	1	Turkish	2024-2025 Spring	Face to Face

Course Coordinator	E-Mail	Phone Number	Course Location	Attendance
Assoc. Prof. Dr. KORAY ŞARKAYA	ksarkaya@pau.edu.tr		FEN A0002	%

Goals		To provide information about understanding of methods of protein specification.
Content		General introduction of protein synthesis, Purity determination methods, Size molecular weight and subunit analysis, Aminoacid analysis, peptide map, cofactor analysis and protein modifications analysis, crystallization methods, radioactive labelling methods, immunological anaysis (Western blot), structure anaysis

Topics

Weeks	Topics
1	General introduction of protein synthesis
2	Purity determination methods
3	Purity determination methods
4	Size molecular weight and subunit analysis
5	Size molecular weight and subunit analysis
6	Aminoacid analysis
7	Peptide map
8	cofactor analysis and protein modifications analysis
9	cofactor analysis and protein modifications analysis
10	crystallization methods
11	radioactive labelling methods
12	immunological anaysis (Western blot)
13	immunological anaysis (Western blot)
14	structure anaysis

Materials

Materials are not specified.

Resources

Resources	Resources Language
D. L. Nelson, M M. Cox, ”Lehninger Principles of Biochemistry”, Worth Publishers, 2000.	English

Course Assessment

Assesment Methods	Percentage (%)	Assesment Methods Title
Final Exam	50	Final Exam
Midterm Exam	50	Midterm Exam

L+P: Lecture and Practice
PQ: Program Learning Outcomes
LO: Course Learning Outcomes

Prospective Student