Weeks | Topics |
1 |
Introduction to basic principles,
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2 |
Aseptic techniques, and the use of the vertical laminar-flow biosafety cabinet.
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3 |
Selection and preparation of media and antibiotics, waste disposal. Freezing and thawing of cells, passaging of adherent cells, passaging floater cells, cell counting and
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4 |
determination of cell viability. Cell viability determination.
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5 |
Differences between primary, transformed, and cancer cells.
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6 |
Establishment of primary cultures from tissue.
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7 |
Separation of mononuclear cells from blood.
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8 |
Methods for determining cell cycling, growth and viability.
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9 |
Cell viability assessment by trypan blue exclusion assay. Cell growth determination by MTT assay.
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10 |
Scallng-up of animal cell cultures.
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11 |
MIDTERM
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12 |
Cell line preservation and authentication.
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13 |
Epithelial stem cell Identification, isolation, and culture.
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14 |
Senescence, apoptosis, and necrosis.
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