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COURSE INFORMATION
Course CodeCourse TitleL+P HourSemesterECTS
BIO 569EXPERIMENTAL ENZYMOLOGY2 + 42nd Semester7,5

COURSE DESCRIPTION
Course Level Master's Degree
Course Type Elective
Course Objective This laboratory course is concerned with molecular and biochemical aspects of enzymology involving purification and structural/functional characterization of enzymes.
Course Content This laboratory course is concerned with molecular and biochemical aspects of enzymology which is the purification and structural/functional characterization of enzymes. Techniques are homogenization, to obtain cell free extract, centrifugation to separate membrane from soluble proteins, initial separations by ammonium sulfate precipitation and purifications by chromatography (anion/cation exchange, hydrophobic interaction, gel filtration, HPLC, and several concentration methods). Assessment protocols such as 1D or 2D gel electrophoresis and, western blotting combined with colorimetric protein concentration determinations, functional characterization with activity assays using UV/VIS and various spectroscopies. For further characterization MALDI-TOF technique will be discussed in theory.
Prerequisites No the prerequisite of lesson.
Corequisite No the corequisite of lesson.
Mode of Delivery Face to Face

COURSE LEARNING OUTCOMES
1Will be able to do homogenization.
2Will be able to perform solubilization of membrane proteins.
3Will be able to carry out purification involving chromaotographic techniques.
4Will be able to perform colorimetric assessments.
5Will be able to carry out 2D Gel electrophoresis.
6Will be able to do activity determinations.
7Will be able to do spectroscopic analyses.

COURSE'S CONTRIBUTION TO PROGRAM
PO 01PO 02PO 03PO 04PO 05PO 06PO 07PO 08PO 09PO 10PO 11
LO 0014433 45 43 
LO 002 344   543 
LO 0035433 445   
LO 0044544       
LO 005  4444     
LO 0064444       
LO 007 444       
Sub Total1724262641291086 
Contribution23441211110

ECTS ALLOCATED BASED ON STUDENT WORKLOAD BY THE COURSE DESCRIPTION
ActivitiesQuantityDuration (Hour)Total Work Load (Hour)
Course Duration (14 weeks/theoric+practical)14342
Hours for off-the-classroom study (Pre-study, practice)14798
Assignments155
Mid-terms11515
Final examination13535
Total Work Load

ECTS Credit of the Course






195

7,5
COURSE DETAILS
 Select Year   


 Course TermNoInstructors
Details 2011-2012 Spring1ALAATTİN ŞEN


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Course Details
Course Code Course Title L+P Hour Course Code Language Of Instruction Course Semester
BIO 569 EXPERIMENTAL ENZYMOLOGY 2 + 4 1 Turkish 2011-2012 Spring
Course Coordinator  E-Mail  Phone Number  Course Location Attendance
Prof. Dr. ALAATTİN ŞEN sena@pau.edu.tr Course location is not specified. %
Goals This laboratory course is concerned with molecular and biochemical aspects of enzymology involving purification and structural/functional characterization of enzymes.
Content This laboratory course is concerned with molecular and biochemical aspects of enzymology which is the purification and structural/functional characterization of enzymes. Techniques are homogenization, to obtain cell free extract, centrifugation to separate membrane from soluble proteins, initial separations by ammonium sulfate precipitation and purifications by chromatography (anion/cation exchange, hydrophobic interaction, gel filtration, HPLC, and several concentration methods). Assessment protocols such as 1D or 2D gel electrophoresis and, western blotting combined with colorimetric protein concentration determinations, functional characterization with activity assays using UV/VIS and various spectroscopies. For further characterization MALDI-TOF technique will be discussed in theory.
Topics
WeeksTopics
1 Introduction
2 Proteins
3 Protein Determinations
4 Enzymes
5 Determinations of enzyme activities
6 Enzyme Purification Techniques I
7 Enzyme Purification Techniques II - Colon Chromatographies
8 Enzyme Purification Techniques III - Affinity Purification
9 Enzyme Purification Techniques IV - Capillary Electrophoresis
10 Enzyme Purification Techniques V - FPLC
11 Enzyme Characterization I - Immunochemical Techniques
12 nzyme Characterization II - Spectrophotometric
13 nzyme Characterization III- Electrophoretic
14 nzyme Characterization IV- Kinetics
Materials
Materials are not specified.
Resources
ResourcesResources Language
Methods in Enzymology Vol 182English
Course Assessment
Assesment MethodsPercentage (%)Assesment Methods Title
Final Exam40Final Exam
Midterm Exam20Midterm Exam
Project40Project
L+P: Lecture and Practice
PQ: Program Learning Outcomes
LO: Course Learning Outcomes